arthyperАртериальная гипертензия"Arterial’naya Gipertenziya" ("Arterial Hypertension")1607-419X2411-8524Antihypertensive League10.18705/1607-419X-2007-13-4-250-255arthyper-1141Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESИзменение уровня мРНК натрий-водородного обменника, натрий-калиевой атфазной помпы, натрий-калий-хлор симпортера и глицеральдегидфосфатдегидрогеназы в лимфоцитах пациентов с мягкой артериальной гипертензийChanges in the level of NHE1, ATP1B1, NKCC1, and GAPDH mRNAs in lymphocytes from patients with mild essential hypertensionВерениновА. А.VeremnovaA. A.noemail@neicon.ruМатвеевВ. В.MatveevaV. V.noemail@neicon.ruГлушанковаЛ. Н.GlushankovaaL. N.noemail@neicon.ruЮринскаяВ. Е.YurinskayaaV. E.noemail@neicon.ruВасильеваИ. О.VassilievaaI. О.noemail@neicon.ruКалюжныйА. А.KalyuznybA. A.noemail@neicon.ruИсаковВ. А.IsakovbV. A.noemail@neicon.ruКовалевЮ. Р.KovalevbY. R.noemail@neicon.ruинститут цитологии РАНРоссияInstitute of Cytology, Russian Academy of SciencesRussian Federationгосударственная педиатрическая медицинская академияРоссияState Medical Academy of Pediatrics, St. Petersburg, RussiaRussian Federation200728082007134250255Copyright © Веренинов А.А., Матвеев В.В., Глушанкова Л.Н., Юринская В.Е., Васильева И.О., Калюжный А.А., Исаков В.А., Ковалев Ю.Р., 20072007Веренинов А.А., Матвеев В.В., Глушанкова Л.Н., Юринская В.Е., Васильева И.О., Калюжный А.А., Исаков В.А., Ковалев Ю.Р.Veremnova A.A., Matveeva V.V., Glushankovaa L.N., Yurinskayaa V.E., Vassilievaa I.О., Kalyuznyb A.A., Isakovb V.A., Kovalevb Y.R.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://htn.almazovcentre.ru/jour/article/view/1141

Цель настоящего исследования - получить новые данные об экспрессии NHE1 мрнК в периферических лим-фоцитах человека у пациентов с артериальной гипертензией и сравнить экспрессию NHE1 мрнК с экспрессией мрнК, кодирующей синтез двух ранее неизученных переносчиков ионов, а также белков несвязанных с ионным транспортом: β-актином, глицеральдегид-фосфатдегидрогеназой (GAPDH), регуляторным антиапоптотическим белком (Bcl-2), и сывороточной глюкокортикоидрегулирующей киназой (hSGK) Meтоды: мрнК выделенная из лимфоцитов периферической крови подвергалась полимеразной цепной реакции (пЦр). Количество кДнК оценивалось методом фотометрии с использованием фотокамеры и компьютерной программы. группа больных включала 26 мужчин в возрасте 18-30 лет с мягкой артериальной гипертензией, группа контроля - лиц с нормаль¬ным АД - 18 сопоставимых мужчин. Уровни NHE1, ATP1B1, NKCC1, GAPDH мрнК, в отличие от Bcl-2 и hSGK, в лимфоцитах больных с Аг были выше, чем у нормотензивных лиц в 2.4-2.6 раза для ионных транспортеров и в 3.5 для GAPDH. ез двух ранее неизученных переносчиков ионов, а также белков несвязанных с ионным транспортом: β-актином, глицеральдегид-фосфатдегидрогеназой (GAPDH), регуляторным антиапоптотическим белком (Bcl-2), и сывороточной глюкокортикоидрегулирующей киназой (hSGK) Meтоды: мрнК выделенная из лимфоцитов периферической крови подвергалась полимеразной цепной реакции (пЦр). Количество кДнК оценивалось методом фотометрии с использованием фотокамеры и компьютерной программы. группа больных включала 26 мужчин в возрасте 18-30 лет с мягкой артериальной гипертензией, группа контроля - лиц с нормаль¬ным АД - 18 сопоставимых мужчин. Уровни NHE1, ATP1B1, NKCC1, GAPDH мрнК, в отличие от Bcl-2 и hSGK, в лимфоцитах больных с Аг были выше, чем у нормотензивных лиц в 2.4-2.6 раза для ионных транспортеров и в 3.5 для GAPDH.

To study whether lymphocytes from patients with mild essential hypertension differ from healthy persons in the levels of mRNAs encoding Na/H exchanger (NHE1), some other ion transporters, i.e. sodium pump (ATP1B1), NaK2Cl symporter (NKCC1), and proteins unrelated to ion transport: glycolytic enzyme (GAPDH), antiapoptotic regulator (BC1-2) and kinase hSGK. Methods: Human peripheral blood lymphocyte mRNAs were assayed by RT PCR procedure. The amount of cDNA was quantified by photometry of electrophoretic bands stained with ethidium bromide, using digital photocamera and PC program. Hypertensive group included 26 males aged 18-30 years with mild essential hypertension, while normotensives -18 males with the same baseline characteristics except that for blood pressure. Results: The levels of NHE1, ATP1B1, NKCC1, GAPDH mRNAs, but not Bcl-2 or hSGK, in lymphocytes from hypertensives are higher than in normotensives by about factor 2.4-2.6 for ion transporters and 3.5 for GAPDH. Conclusions: The lymphocytes from young males with mild hypertension differ from normotensive ones in that there is a change in abundance of a group of mRNAs comprising not only NHE1, but either ATP1B1, NKCC1, and GAPDH mRNAs. The NHE1 effect is not specific.

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The authors declare that there are no conflicts of interest present.