arthyperАртериальная гипертензия"Arterial’naya Gipertenziya" ("Arterial Hypertension")1607-419X2411-8524Antihypertensive League10.18705/1607-419X-2020-26-6-656-664arthyper-1941Research ArticleСтатьиArticlesВзаимосвязь дефицита фолатов, гипергомоцистеинемии и метаболизма глутатиона у больных артериальной гипертензиейThe relationship of folate deficiency, hyperhomocysteinemia and glutathione metabolism in hypertensive patientsАлександроваЛ. А.AleksandrovaL. A.

Александрова Людмила Александровна — кандидат биологических наук, старший научный сотрудник отдела биохимии Научно-образовательного института биомедицины

ул. Л. Толстого, д.6–8, Санкт-Петербург, 197022

Lyudmila A. Aleksandrova, PhD in Biology Sciences, Senior Researcher, Biochemistry Department, Research Educational Institute of Biomedicine

6–8 L. Tolstoy street, St Petersburg, 197022

Laa2004@mail.ruСубботинаТ. Ф.SubbotinaT. F.

Субботина Татьяна Федоровна —доктор медицинских наук, профессор, руководитель лаборатории биохимического мониторинга отдела биохимии Научно-образовательного института биомедицины

Санкт-Петербург

Tatyana F. Subbotina, MD, PhD, DSc, Professor, Head, Laboratory of Biochemical Monitoring, Biochemistry Department, Research Educational Institute of Biomedicine

St Petersburg

subbotina2002@mail.ruЖлобаА. А.ZhlobaA. A.

Жлоба Александр Анатольевич — доктор медицинских наук, профессор, руководитель отдела биохимии Научно-образовательного института биомедицины

Санкт-Петербург

Aleksandr A. Zhloba, MD, PhD, DSc, Professor, Head, Biochemistry Department, Research Educational Institute of Biomedicine

St Petersburg

zhloba@mail.spbnit.ruПервый Санкт-Петербургский государственный медицинский университет имени академика И. П. Павлова, Санкт-Петербург, РоссияРоссияPavlov UniversityRussian FederationГосударственное бюджетное образовательное учреждение высшего профессионального образования «Первый Санкт-Петербургский государственный медицинский университет имени академика И.П. Павлова» Министерства здравоохранения Российской ФедерацииРоссияPavlov UniversityRussian Federation202026112020266656664Copyright © Александрова Л.А., Субботина Т.Ф., Жлоба А.А., 20212021Александрова Л.А., Субботина Т.Ф., Жлоба А.А.Aleksandrova L.A., Subbotina T.F., Zhloba A.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://htn.almazovcentre.ru/jour/article/view/1941

Актуальность. Артериальная гипертензия (АГ) нередко сопровождается дефицитом фолиевой кислоты (ФК) и гипергомоцистеинемией (ГГЦ). Глутатион восстановленный (ГлВ) и зависимые от него ферменты определяют состояние клеточной антиоксидантной и окислительно-восстановительной систем при сердечно-сосудистой патологии. Цель работы — оценить взаимосвязь статуса ФК и наличия ГГЦ с ферментами метаболизма глутатиона и окислительно-восстановительным состоянием глутатиона эритроцитов при АГ. Материалы и методы. В образцах крови от 43 больных АГ, находившихся на стационарном лечении в клиниках ПСПбГМУ им. И. П. Павлова, определяли концентрацию ФК, общего гомоцистеина (оГци) в плазме, а также содержание ГлВ, активность глутатионпероксидазы и глутатионредуктазы (ГР) в эритроцитах. Результаты. В основной группе активность ГР положительно коррелировала с концентрацией ФК (R = 0,415; p = 0,001). Выявлено статистически значимое снижение активности ГР (в Ед/г Hb) в подгруппе с пониженным уровнем ФК [0,8 (0,5–1,1)] по сравнению с подгруппой без дефицита ФК [1,2 (0,9–2,0)]. Уровень ГлВ (в мкМ/г Hb) был также ниже (p < 0,018) в подгруппе с недостаточностью ФК [1,3 (0,9–2,1)] по сравнению с подгруппой с нормальным уровнем ФК [1,8 (1,5–4,6)]. Установлено статистически значимое снижение уровня ГлВ и активности ГР в подгруппе с ГГЦ по сравнению с соответствующими параметрами в подгруппе без ГГЦ. Однако даже в отсутствие ГГЦ у больных с дефицитом ФК обнаружено статистически значимое понижение активности ГР по сравнению с больными без дефицита ФК. При этом ГР положительно коррелировала с ФК (R = 0,564; p = 0,03). Заключение. Дефицит ФК может усиливать недостаточность активности ГР независимо от уровня оГци. Показатель активности ГР в эритроцитах может рассматриваться как возможный маркер функционального дефицита ФК в отсутствии ГГЦ.

Hypertension (HTN) is often accompanied by folic acid (FA) deficiency and hyperhomocysteinemia (HHcy). Reduced glutathione (GSH) and dependent enzymes determine the state of cellular antioxidant and redox systems in cardiovascular pathology. The aim of our work is to assess the relationship between the status of FA and the presence of HHcy with enzymes of glutathione metabolism and the redox state of erythrocyte glutathione in HTN. Design and methods. In blood plasma samples from 43 HTN patients admitted to the clinic of Pavlov University, the concentration of FA and total homocysteine (oHcy) was determined. We also evaluated the level of GSH, the activity of glutathione peroxidase and glutathione reductase (GR) in erythrocytes. Results. In the whole group, GR activity positively correlated with the concentration of FA (R = 0,415; p = 0,001). A significant decrease in GR activity (U/g Hb) was found in the subgroup with the low level of FA [0,8 (0,5–1,1)] compared with the subgroup without a FA deficiency [1,2 (0,9–2,0)]. The GSH level (μM/g Hb) was also lower (p < 0,018) in the subgroup with FA deficiency [1,3 (0,9–2,1)] compared with the subgroup with normal FA levels [1,8 (1,5–4,6)]. A significant decrease in the level of GSH and GR activity in the subgroup with HHcy was found compared with the corresponding parameters in the subgroup without HHcy. However, even in the absence of HHcy patients with FA deficiency demonstrated a significant decrease in GR activity compared to patients without FA deficiency. In this case, GR positively correlated with FA (R = 0,564; p = 0,03). Conclusions. The deficiency of FA can increase the deficiency of GR activity, regardless of the level of oHcy. The indicator of GR activity in erythrocytes can be considered as a possible marker of functional deficiency of FA in the absence of HHcy.

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The authors declare that there are no conflicts of interest present.