The mechanisms of Ca2+-dependent hypertension formation on the model of rat cardiomyocytes in the culture
https://doi.org/10.18705/1607-419X-2009-15-6-683-687
Abstract
Objective. To study the ryanodine (RyR) and dihydropyridine (DHPR) receptor physiological activity in cardiomyocytes of spontaneously hypertensive (SHR) and normotensive (WKY, Wistar) rats aged 0-6 weeks. Design and methods. The receptor activity was estimated by the elevation rate of intracellular Ca2+ concentration ([Ca2+]i) in the cardiomyocyte culture (5 days): in response to 4-chloro-m-cresol (4-CmC) action for RyRs and to Bay K8644 (BayK) one - for DHPR. Results. In response to 4-CmC (2 mM), a drastic increase in the rate of [Ca2+]i accumulation (2,9 ± 0,8 times) was registered in SHR myocytes cultivated for 3 and more weeks versus a decrease in the rates of Ca2+ release via the RyRs of Wistar and WKY rat cardiomyocytes. BayK (80 μM) also induced more sharp [Ca2+]i elevation in SHR cardiomyocytes (3,4 ± 0,3-fold increase) as compared to age-matched Wistar (2,3 ± 0,2-fold increase) and WKY (1,2 ± 0,1-fold increase) ones. Conclusion. In SHR and WKY cardiomyocytes, as compared to normotensive Wistar rats, gradual growth of DHPR activity is observed, which follows by Ca2+-induced Ca2+ release (CICR) (3 weeks) and drastic activation of RyR in SHR myocytes at the end of prehypertensive period (6 weeks).
About the Authors
E. A. Zakharov
Sechenov Institute of Evolutionary Physiology and Biochemistry of RAS
Russian Federation
Russian Federation
N. Z. Klyueva
Pavlov Institute of Physiology of RAS
Russian Federation
Russian Federation
G. B. Belostotskaya
Sechenov Institute of Evolutionary Physiology and Biochemistry of RAS
Russian Federation
Russian Federation
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Review
For citations:
Zakharov E.A., Klyueva N.Z., Belostotskaya G.B. The mechanisms of Ca2+-dependent hypertension formation on the model of rat cardiomyocytes in the culture. "Arterial’naya Gipertenziya" ("Arterial Hypertension"). 2009;15(6):683-687. (In Russ.) https://doi.org/10.18705/1607-419X-2009-15-6-683-687
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