Endothelial functions and DNA structure polymorphism in females with coronary artery disease without angiography verified coronary stenoses

Objective. To assess endothelial function in women with coronary artery disease without angiographic sings of coronary stenoses, considering structural features of DNA. Design and methods. 38 women of 34-80 years old with stable angina pectoris I-III functional class were examined. Myocardial ischemia was confirmed in all patients, and angiographic signs of coronary atherosclerotic lesions were absent. Endothelial function was assessed by measuring flow-mediated dilation and nitroglycerin-mediated dilation of the brachial artery using high-resolution ultrasound. Polymorphisms of genes of endothelial NO-synthase (T-786C, 4a/b, G894T polymorphisms); endothelin-1 (G198T polymorphism), β1-adrenoreceptor (C/G polymorphism) were investigated by polymerase chain reaction. Results. The high frequency of flow-mediated dilation damage, including the paradoxical vasoconstriction reaction, was defined. Nitroglycerin-mediated dilation was normal in all patients. The association the paradoxical vasoconstriction reaction with T-786C and G894T polymorphism of the gene NOS3, G198T gene polymorphism of EDN1, combined genotypes T-786T/G198T, C-786T/G189T, C-786C/G198T, ab/G198T, bb/G189T, G894G/G198G, G894T/G198T, T894T/G189T NOS3 gene and EDN1, С-786С/ С389G gene NOS3 and ADRB1 was found.

coronary artery disease, endothelial dysfunction, structural polymorphism of DNA

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