NAP-22 and MARCKS mRNA expression levels in spontaneously hypertensive rat kidneys: the effect of drinking water with different calcium contents
https://doi.org/10.18705/1607-419X-2021-27-4-436-445
Abstract
Objective. The aim of study was to assess the effect of the level of calcium intake with drinking water on NAP-22 and MARCKS mRNA expression in cortical and medullar kidney layers of spontaneously hypertensive rats.
Design and methods. The study involved 90-day-old SHR (n = 8) and WKY (n = 8) strain rats of both sexes. We assessed tissue samples from cortical and medullar kidney layers. NAP-22 and MARCKS mRNA expression levels were determined by RT-PCR.
Results. Sufficient drinking water calcium intake was associated with similar the expression of NAP-22 and MARCKS mRNA in kidneys of spontaneously hypertensive and normotensive rats. Consumption of drinking water with insufficient calcium content it decreases in both rat strains, being more evident in spontaneously hypertensive rats, especially in the medullar layer.
Conclusions. Our results show that genetically determined impairments of calcium metabolism in cells of spontaneously-hypertensive rats (SHR line) and their effect on intracellular signaling processes are more evident with the reduced intake of exogenous calcium.
Supporting agencies: The work was carried out in the Institute for Analytical Instrumentation, Russian Academy of Sciences within the framework of the Governmental Task 075–00780–19–02, the topic № 0074–2019–0013 of the Ministry of Science and Higher Education of the Russian Federation
About the Authors
A. S. Aldekeeva
Institute for Analytical Instrumentation, Russian Academy of Sciences
Russian Federation
Russian Federation
Anna S. Aldekeeva, Engineer of the 1st Category, Laboratory of Methods and Devices of Immune and Genetic Analysis.
St Petersburg
A. Y. Plekhanov
Petersburg Nuclear Physics Institute, Kurchatov Centre; Institute of Macromolecular Compounds, Russian Academy of Sciences
Russian Federation
Russian Federation
Anton Y. Plekhanov, PhD in Biology Sciences, Researcher, Laboratory of Biopolymers.
Gatchina, St Petersburg
N. Z. Klyueva
Pavlov Institute of Physiology, Russian Academy of Sciences
Russian Federation
Russian Federation
Natalya Z. Klyueva, PhD in Biology Sciences, Senior Researcher, Experimental Cardiology Group.
St Petersburg
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For citations:
Aldekeeva A.S., Plekhanov A.Y., Klyueva N.Z. NAP-22 and MARCKS mRNA expression levels in spontaneously hypertensive rat kidneys: the effect of drinking water with different calcium contents. "Arterial’naya Gipertenziya" ("Arterial Hypertension"). 2021;27(4):436-445. (In Russ.) https://doi.org/10.18705/1607-419X-2021-27-4-436-445
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