A603G tissue factor gene polymorphism and mediators of immune inflammation in patients with arterial hypertension and coronary heart disease

Background. Genetic factors have clinical significance in arterial hypertension and coronary heart disease (CHD). Objective. To assess A603G tissue factor gene polymorphism and mediators of immune inflammation in patients with arterial hypertension and СHD. Design and methods. The A603G genotype was determined in a sample of 105 patients with arterial hypertension and CHD by a polymerase chain reaction-restriction length polymorphism (PCR-RFLP)-based method. We studied the mediators of immune inflammation — interleukin-8 (IL-8), interferon-gamma (IF-gamma), tumor necrosis factor-alpha (TNF) and interleukin-10 (IL-10). Results. 24 patients (13,3 %) were the carriers of AA genotype, patients had AG and GG genotype. CHD was diagnosed at the age of 52,1 ± 2,48 years in patients with GG genotype compared to 59,2 ± 2,54 years in AA genotype group and 60,1 ± 1,84 years in AG genotype group (p < 0,05) . Levels of IL-8 (9,81 ± 2,14 pkg/ml) and IF-gamma (1,58 ± 0,25 pkg/ml) were elevated in patients with GG genotype in comparison with AA genotype patients (3,08 ± 0,59 и 0,99 ± 0,21 pkg/ml, respectively, p < 0,05). Conclusion. Our data support that GG genotype of tissue factor in patients with arterial hypertension is associated with early debut of CHD and activation of proin-flammatory cytokines, mediators of endothelial dysfunction.

tissue factor, immune inflammation, arterial hypertension, coronary heart disease

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